Premium
Synthesis, Structure, DNA‐Binding Properties, and Cytotoxicity of Ruthenium(II) Polypyridyl Complexes
Author(s) -
Liu YunJun,
Zeng ChengHui,
Yao JunHua,
Wu FuHai,
He LiXin,
Huang HongLiang
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200900213
Subject(s) - chemistry , ruthenium , singlet oxygen , cytotoxicity , dna , phenanthroline , cleavage (geology) , bromide , stereochemistry , titration , medicinal chemistry , in vitro , oxygen , crystallography , inorganic chemistry , biochemistry , catalysis , organic chemistry , geotechnical engineering , fracture (geology) , engineering
Many ruthenium(II) complexes show high antitumor activities, and the in vitro antitumor activities are usually related to DNA binding. We designed and synthesized two Ru II polypyridyl complexes, [Ru(dmp) 2 (fpp)] 2+ (dmp=2,9‐dimethyl‐1,10‐phenanthroline; fpp=2‐[3,4‐(difluoromethylenedioxy)phenyl]imidazo[4,5‐ f ] [1,10]phenanthroline and [Ru(phen) 2 (fpp)] 2+ (phen=1,10‐phenanthroline). The DNA‐binding properties of these complexes have been investigated by spectroscopic titration, DNA melting experiments, viscosity measurements, and photoactivated cleavage. The mechanism studies of photocleavage revealed that singlet oxygen ( 1 O 2 ) and superoxide anion radical (O $\rm{{_{2}^{{^\cdot} -}}}$ ) may play an important role in the photocleavage. The cytotoxicity of complexes 1 and 2 have been evaluated by MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2 H ‐tetrazolium bromide) method; complex 2 shows slightly higher anticancer potency than 1 does against all the cell lines screened.