The Chemical Constituents of Endophytic Fungus Trichoderma sp. MFF‐1

A fungal strain named MFF‐1 was isolated from the flower of Pyrethrum cinerariifolium. Based on the sequence at the internal transcribed spacer (ITS) region, this strain was identified as a Trichoderma sp. Two new compounds, including a mitorubrin derivative and its potential biogenetic precursor, together with a known compound, were isolated from the cultures of the endophytic fungus. Their structures were established by spectroscopic methods and determined to be (3 S* ,6 R* ,7 R* )‐3,4,5,6,7,8‐hexahydro‐7‐hydroxy‐7‐methyl‐8‐oxo‐3‐[( E )‐prop‐1‐enyl]‐1 H ‐isochromen‐6‐yl 2,4‐dihydroxy‐6‐methylbenzoate ( 1 ), named deacetylisowortmin, ( E )‐2‐(hydroxymethyl)‐3‐(2‐hydroxypent‐3‐enyl)phenol ( 2 ), and wortmannin ( 3 ). All compounds were assayed for antimicrobial activity. Compound 3 showed activity against Candida albicans and Bacillus cereus.