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Structural Characterization of Lipopeptide Methyl Esters Produced by Bacillus licheniformis HSN 221
Author(s) -
Li Yiming,
Yang Shizhong,
Mu Bozhong
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200900155
Subject(s) - bacillus licheniformis , lipopeptide , surfactin , chemistry , fatty acid , bacillus subtilis , bacillales , chromatography , bacillaceae , bacteria , biochemistry , stereochemistry , biology , genetics
Lipopeptides and their analogues are of increasing interest due to their amphiphilic structures and potential applications in various fields. Three purified lipopeptides analogues were obtained at the same time after two‐step column‐chromatographic purification from cell‐free broth cultivated by Bacillus licheniformis HSN 221. Analysis by ESI‐MS, GC/MS, HPLC, and Q‐TOF MS/MS revealed their primary structures as anteiso ‐C 15 ‐ and iso ‐C 15 ‐ β ‐hydroxy fatty acid‐Gln‐Leu‐Leu‐Val‐MeAsp‐Leu‐Ile, anteiso ‐C 15 ‐ and iso ‐C 15 ‐ β ‐hydroxy fatty acid‐MeGlu‐Leu‐Leu‐Val‐Asp‐Leu‐Ile and iso ‐C 16 ‐ β ‐hydroxy fatty acid‐Glu‐Leu‐Leu‐Val‐MeAsp‐Leu‐Ile, respectively. The production of two surfactin monomethyl esters and one lichenysin monomethyl ester directly from microorganisms is helpful to understand the variants of metabolites.