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Characterization of the Posttranscriptional Modifications in Legionella pneumophila Small‐Subunit Ribosomal RNA
Author(s) -
Emmerechts Gert,
Maes Liesbeth,
Herdewijn Piet,
Anné Jozef,
Rozenski Jef
Publication year - 2008
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200890218
Subject(s) - legionella pneumophila , ribosomal rna , protein subunit , chemistry , characterization (materials science) , rna , microbiology and biotechnology , biochemistry , bacteria , biology , genetics , gene , nanotechnology , materials science
Abstract It is generally accepted that posttranscriptional modifications in RNA play a role in the fine‐tuning of RNA function and the maintenance of RNA structure. This article describes the characterization of the posttranscriptional modifications in Legionella pneumophila 16S rRNA by mass spectrometry and reverse transcriptase assays. Eight modified nucleotides were identified and mapped in the 16S rRNA sequence. Situation of these data in relation to general 16S rRNA modification patterns shows that L. pneumophila is relatively less modified, and that the majority of the L. pneumophila 16S rRNA modifications are conserved among the bacteria characterized so far ( Escherichia coli, Clostridium acetobutylicum, Thermus thermophilus , and Thermotoga maritima ).