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Metal‐Induced Point Defects in DNA: Model and Mechanisms
Author(s) -
Bregadze Vasil G.,
Gelagutashvili Eteri S.,
Tsakadze Ketevan J.,
Melikishvili Sophia Z.
Publication year - 2008
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200890182
Subject(s) - chemistry , tautomer , dna , base pair , depurination , crystallography , rna , ion , point mutation , stereochemistry , guanine , metal ions in aqueous solution , helix (gastropod) , nucleobase , tryptophan , amino acid , mutation , nucleotide , biochemistry , organic chemistry , ecology , biology , snail , gene
The aim of this work was to study the role of H 3 O + and transition‐metal (TM) ions in keto–enol and amino–imino tautomeric transitions in DNA base pairs and depurination. In this regard, we discuss the thermodynamic model of ion–DNA interactions and UV display of double‐proton transfer (DPT) in GC. The probabilities and energies of rare tautomeric forms of GC pairs in DNA induced by H 3 O + and TM were determined being in the range from 0.02 (for Mg 2+ ) to 1 (for Cu 2+ ), and from 0 kcal/ M (for Cu + ) to 2.3 kcal/ M (for Mg 2+ ), respectively. It was shown that site of DNA double helix, which corresponds to the only triplet 5′ UGG 3′ of RNA that codes the most valuable amino acid tryptophan, is a good target for TM ions to attack. It was also shown that the only way to obtain the tryptophan‐coding 5′ UGG 3′ triplet in RNA via transition‐type G→A point mutation caused by TM ions is their interaction with the site of a DNA double helix, which corresponds to 5′ CGG 3′ triplet of RNA that codes arginine.