Constituents of  Asarum sieboldii  with Inhibitory Activity on Lipopolysaccharide (LPS)‐Induced NO Production in BV‐2 Microglial Cells | Zendy

Han AhReum | Zendy; Kim Hye Jeoung | Zendy; Shin Minkyu | Zendy; Hong Moochang | Zendy; Kim Yang Seok | Zendy; Bae Hyunsu | Zendy

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Constituents of Asarum sieboldii with Inhibitory Activity on Lipopolysaccharide (LPS)‐Induced NO Production in BV‐2 Microglial Cells

Author(s) -

Han AhReum,

Kim Hye Jeoung,

Shin Minkyu,

Hong Moochang,

Kim Yang Seok,

Bae Hyunsu

Publication year - 2008

Publication title -

chemistry and biodiversity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.427

H-Index - 70

eISSN - 1612-1880

pISSN - 1612-1872

DOI - 10.1002/cbdv.200890033

Subject(s) - sesamin , chemistry , lipopolysaccharide , safrole , nitric oxide , in vitro , inhibitory postsynaptic potential , bioassay , fractionation , stereochemistry , biochemistry , chromatography , food science , biology , organic chemistry , endocrinology , neuroscience , genetics

Bioassay‐guided fractionation of the root extract of Asarum sieboldii led to the isolation of the four active compounds (−)‐sesamin ( 1 ), (2 E ,4 E ,8 Z ,10 E )‐ N ‐(2‐methylpropyl)dodeca‐2,4,8,10‐tetraenamide ( 2 ), kakuol ( 3 ), and ‘3,4,5‐trimethoxytoluene’ (=1,2,3‐trimethoxy‐5‐methylbenzene; 4 ), in terms of inhibition of lipopolysaccharide (LPS)‐induced nitric oxide (NO) production. Compounds 1 – 4 showed potent inhibition of NO production, with IC 50 values in the low nanomolar‐to‐micromolar range. Also isolated were the known compounds methylkakuol ( 5 ), ‘3,5‐dimethoxytoluene’, safrole, asaricin, methyleugenol, and (−)‐asarinin, which were found to be inactive in the above assay. Among the ten known isolates, compounds 1, 2 , and 5 were found for the first time in this plant.

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