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Enzymatically Catalyzed DNA Synthesis Using L ‐Asp‐dGMP, L ‐Asp‐dCMP, and L ‐Asp‐dTMP
Author(s) -
Terrazas Montserrat,
Marlière Philippe,
Herdewijn Piet
Publication year - 2008
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200890013
Subject(s) - chemistry , pyrophosphate , moiety , nucleic acid , catalysis , aspartic acid , stereochemistry , dna , enzyme , biochemistry , combinatorial chemistry , amino acid
The replacement of the pyrophosphate moiety of deoxynucleoside triphosphates by L ‐aspartic acid allows incorporation of natural deoxynucleosides into DNA using HIV reverse transcriptase (RT) as enzyme, while retaining the canonical base‐pair selectivity. N ‐Methylation of the L ‐aspartic acid leaving group results in a reduced fidelity of incorporation.