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Synthesis of Oligonucleotide Derivatives Using ChemMatrix Supports
Author(s) -
Mazzini Stefania,
GarcíaMartin Fayna,
Alvira Margarita,
Aviñó Anna,
Manning Brendan,
Albericio Fernando,
Eritja Ramon
Publication year - 2008
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200890012
Subject(s) - oligonucleotide , chemistry , combinatorial chemistry , oligonucleotide synthesis , ethylene glycol , anhydrous , conjugate , dna , organic chemistry , biochemistry , mathematical analysis , mathematics
The synthesis of oligonucleotides on poly(ethylene glycol)‐based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthesis of oligonucleotide–peptide conjugates and for the ligation of oligonucleotides using Cu + ‐catalyzed cycloadition reactions is reported. Moreover, these supports can be used for the preparation of oligonucleotides in anhydrous solvents, followed by hybridization of the complementary sequences in aqueous buffers.