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Casearin X, Its Degradation Product and Other Clerodane Diterpenes from Leaves of Casearia sylvestris: Evaluation of Cytotoxicity against Normal and Tumor Human Cells
Author(s) -
Gonzaga dos Santos André,
Pinheiro Ferreira Paulo Michel,
Magela Vieira Júnior Gerardo,
Perez Carla Cristina,
Gomes Tininis Aristeu,
Silva Geraldo Humberto,
da Silva Bolzani Vanderlan,
Veras CostaLotufo Letícia,
do Ó Pessoa Cláudia,
Cavalheiro Alberto José
Publication year - 2010
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800342
Subject(s) - diterpene , chemistry , cytotoxic t cell , cytotoxicity , stereochemistry , cancer cell , cell culture , cancer cell lines , biochemistry , cancer , biology , in vitro , genetics
An EtOH extract of the leaves of Casearia sylvestris afforded new clerodane diterpene, casearin X, together with the known compounds casearins B, D, L, and O, and caseargrewiin F. Casearin X degraded to the corresponding dialdehyde when stored in CDCl 3 . The diterpenes isolated were cytotoxic to human cancer cell lines, with caseargrewiin F being the most active and the new clerodane, casearin X, the second active compound with IC 50 values comparable to the positive control doxorubicin. All isolated diterpenes showed lower activities against normal human cells than against cancer cell lines, which might indicate a possible selective action on cancer cells. Casearin X dialdehyde was not cytotoxic to cancer cells indicating that the occurrence of these CO groups at C(18) and C(19) is incompatible with the cytotoxic activity.