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Degradation of High‐Molar‐Mass Hyaluronan by Ascorbate plus Cupric Ions: Effects of D ‐Penicillamine Addition
Author(s) -
Valachová Katarína,
Rapta Peter,
Kogan Grigorij,
Hrabárová Eva,
Gemeiner Peter,
Šoltés Ladislav
Publication year - 2009
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800261
Subject(s) - chemistry , radical , electron paramagnetic resonance , penicillamine , ascorbic acid , molar mass , kinetics , degradation (telecommunications) , macromolecule , radiolysis , nuclear chemistry , decomposition , photochemistry , hyaluronic acid , organic chemistry , biochemistry , polymer , telecommunications , physics , food science , nuclear magnetic resonance , quantum mechanics , biology , computer science , genetics
Pro‐ and anti‐oxidative effects of an anti‐rheumatoid drug, D ‐penicillamine ( D ‐PN), on the kinetics of high‐molar‐mass hyaluronan (HA) degradation were monitored using the method of rotational viscometry. The degradation of the dissolved HA macromolecules was attained by applying the Weissberger 's system comprising ascorbic acid plus cupric ions. Electron paramagnetic resonance (EPR) spectroscopy was used to identify the generated free radicals. The results obtained indicate that the initial anti‐oxidative action of D ‐PN is followed by induction of pro‐oxidative conditions due to the generation of reactive free radicals. It is speculated, however, that the latter situation may be considered as an advantageous property of D ‐PN. Hydroxyl radicals formed in this way may participate in decomposition of proteinases, which are believed to be responsible for the destruction of joint cartilage under rheumatoid arthritic conditions.