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Antiproliferation and Apoptosis Induced by Evodiamine in Human Colorectal Carcinoma Cells (COLO‐205)
Author(s) -
Yang ZhiGang,
Chen AQin,
Liu Bo
Publication year - 2009
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800256
Subject(s) - evodiamine , apoptosis , agarose gel electrophoresis , chemistry , dna fragmentation , mtt assay , microbiology and biotechnology , cell cycle , flow cytometry , cell growth , fluorescence microscope , blot , fragmentation (computing) , programmed cell death , biochemistry , biology , dna , fluorescence , ecology , physics , chromatography , quantum mechanics , gene
Evodiamine ( 1 ), a biologically active alkaloid isolated from Evodia rutaecarpa (known in Chinese as Wu‐Chu‐Yu ), has antioxidant, anti‐inflammatory, and anticancer activities. It has recently been demonstrated that the cytotoxic activities of 1 might be due to its ability to inhibit cell growth and induce apoptosis. In this study, we investigated the effects of 1 on growth and apoptosis in COLO‐205 cells by MTT assay, fluorescence microscopy, transmission electron microscopy, DNA fragmentation assay, flow cytometry, immunohistochemical analysis, Western blotting, and caspase‐3 activity assay. Our data revealed that 1 could significantly inhibit COLO‐205 cell proliferation in a dose‐dependent manner, and 1 ‐treated COLO‐205 cells displayed typical morphological apoptotic characteristics and formation of DNA ladders in agarose gel electrophoresis. The COLO‐205 cell cycle was arrested in G 2 /M phase by 1 . Meanwhile, 1 increased the expression of Bax and p53, decreased the expression of Bcl‐2, lowered the mitochondrial transmembrane potential, and induced the activation of caspase‐3. These activities may contribute to the anticarcinogenic action of 1 .