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Stemucronatoside L, a Pregnane Glycoside from the Roots of Stephanotis mucronata , Inhibits Th1/Th2 Immune Responses in vitro
Author(s) -
Chen FengYang,
Ye YiPing,
Sun HongXiang,
Li XiaoYu,
Shi Hong
Publication year - 2009
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800159
Subject(s) - immune system , chemistry , in vitro , cd8 , splenocyte , messenger rna , interleukin 4 , microbiology and biotechnology , transcription (linguistics) , transcription factor , immunology , biology , gene , biochemistry , linguistics , philosophy
temucronatoside L (SML), isolated from Stephanotis mucronata , could suppress the activation of T cells in vitro. However, the mechanisms responsible for its immunosuppressive activity remain poorly understood. The purpose of this study was to investigate whether SML could suppress Th1/Th2 immune responses and to characterize the cellular mechanisms involved. Effects of SML on T‐lymphocyte subsets and the production of Th1 cytokines IL‐2 and IFN‐ γ , and Th2 cytokines IL‐4 and IL‐10 from ConA‐stimulated mice splenocytes were detected by flow‐cytometric analysis and ELISA method, respectively. Furthermore, effects of SML on mRNA expression level of Th1/Th2 cytokines and transcription factors T‐bet and GATA‐3 were evaluated by RT‐PCR analysis. SML not only significantly decreased the percentage of CD4 + T cells and the CD4 + /CD8 + ratio, but reduced the production of Th1/Th2 cytokines in a concentration‐dependent manner. The mRNA expression levels of Th1/Th2 cytokines and transcription factors (T‐bet and GATA‐3) were also suppressed by SML. These results suggested that SML could simultaneously inhibit Th1/Th2 immune responses by suppressing gene expression of Th1/Th2 cytokines and transcription factors.