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Cyclen‐Based Side‐Chain Homopolymer Self‐Assembly with Plasmid DNA: Protection of DNA from Enzymatic Degradation
Author(s) -
Li Kun,
Wang Na,
Yang Fan,
Zhang ZhongWei,
Zhou LiHong,
Chen ShanYong,
Lin HongHui,
Tian YunFei,
Yu XiaoQi
Publication year - 2009
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800080
Subject(s) - cyclen , chemistry , dna , circular dichroism , polymer , polyelectrolyte , biophysics , plasmid , degradation (telecommunications) , side chain , enzyme , crystallography , biochemistry , stereochemistry , organic chemistry , telecommunications , computer science , biology
In this study, a 1,4,7,10‐tetraazacyclododecane (cyclen)‐based side‐chain homopolymer was developed for the first time; this polymer can self‐assembly with plasmid DNA to form polyelectrolyte complexes (polyplex), which can protect DNA from enzymatic degradation. Moreover, the polyplex can disassembly and release free DNA when NaCl solution is added to this system. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) are used for imaging of the surface structure of the polyplex, and results indicated that the polyplex structures respond to the polymer concentration. Circular dichroism (CD) spectrum suggested that the DNA configuration in the polyplex was retained.