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A 1 : 2 Copper(II)–Tripeptide Complex for DNA Binding and Cleavage Agent under Physiological Conditions
Author(s) -
Reddy Pulimamidi R.,
Manjula Pallerla,
Chakraborty Tushar K.,
Samanta Rajarshi
Publication year - 2009
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200800079
Subject(s) - chemistry , tripeptide , cleavage (geology) , copper , dna , biochemistry , stereochemistry , biophysics , combinatorial chemistry , peptide , organic chemistry , fracture (geology) , biology , geotechnical engineering , engineering
A 1 : 2 copper–tripeptide complex, [Cu II (Boc‐His‐Gly‐His‐OMe) 2 ] 2+ , was synthesized and structurally characterized. The absorption band at 577 nm suggests a square‐planar geometry around Cu II . The DNA‐binding and DNA‐cleavage properties of the Cu II complex were investigated. The complex binds to calf thymus DNA (CT DNA) in an intercalative fashion and cleaves plasmid pUC‐19 DNA hydrolytically at micromolar concentrations under physiological conditions. The intrinsic binding constant ( K b =1.2×10 2 M −1 ) for the binding of Cu–tripeptide complex with DNA suggests that this complex is suitable for rapid diffusion on the pharmacological time scale.