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Nonenzymatic Recombination of RNA: Possible Mechanism for the Formation of Novel Sequences
Author(s) -
Lutay Alexei V.,
Zenkova Marina A.,
Vlassov Valentin V.
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790062
Subject(s) - phosphodiester bond , rna , chemistry , oligonucleotide , recombination , biophysics , biochemistry , dna , gene , biology
Abstract We report on the formation of novel RNA molecules in a recombination‐like, nonenzymatic reaction proceeding in the complex of partially complementary RNA‐oligonucleotides under very simple conditions. Analysis of the isolated products demonstrated that at least 5% of the formed linkages are of the (natural) 3′,5′‐phosphodiester type. We suggest that similar reactions could contribute to the development of the ‘RNA world’, but could also proceed in vivo within variously structured RNA or RNA complexes containing loops, bulges, or dangling ends, providing an emergence of novel RNA sequences.