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Chemical and Genetic Differentiation of Ligularia tsangchanensis in Yunnan and Sichuan Provinces of China
Author(s) -
Torihata Atsushi,
Hanai Ryo,
Gong Xun,
Shen Yuemao,
Kuroda Chiaki
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790042
Subject(s) - china , sichuan basin , genetic diversity , intraspecific competition , clade , internal transcribed spacer , ribosomal rna , gene , geography , biology , zoology , genetics , phylogenetics , demography , geology , archaeology , population , geochemistry , sociology
Abstract The intraspecific diversity in L. tsangchanensis collected in the Chinese Provinces Yunnan and southwestern Sichuan was studied by chemical and genetic approaches. The samples collected in Yunnan were found to contain cacalol ( 1 ) as the sole major component, while samples from Sichuan contained 7 α ‐ and 7 β ‐eremophila‐9,11‐dien‐8‐one ( 5 and 6 ) as well as the 3 α ‐angeloyloxy derivative 7 as major components. In addition, the sequences of the internal transcribed spacers (ITSs) of the ribosomal RNA gene indicated that the Yunnan and the Sichuan samples constitute separate clades. These results demonstrate that L. tsangchanensis in Yunnan and Sichuan are distinct.