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Immunological Adjuvant Effect of Ginsenoside Rh 4 from the Roots of Panax notoginseng on Specific Antibody and Cellular Response to Ovalbumin in Mice
Author(s) -
Yang ZhiGang,
Ye YiPing,
Sun HongXiang
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790028
Subject(s) - ovalbumin , panax notoginseng , adjuvant , chemistry , concanavalin a , saponin , splenocyte , antibody , ginsenoside , lipopolysaccharide , haemolysis , pharmacology , antibody response , andrology , immunology , immune system , microbiology and biotechnology , biochemistry , ginseng , biology , medicine , in vitro , alternative medicine , pathology
Ginsenoside Rh 4 ( 1 ), a saponin isolated from the roots of Panax notoginseng ( Burk .) F. H. Chen , was evaluated for its haemolytic activity and adjuvant potential on specific antibody and cellular response to ovalbumin (OVA) in mice. Compound 1 showed a slight haemolytic effect, its concentration inducing 50% of the maximum haemolysis ( HD 50 value) being 407±12 μg/ml using a 0.5% suspension of red blood cells. Compound 1 significantly increased the concanavalin A (Con A)‐, lipopolysaccharide (LPS)‐, and OVA‐induced splenocyte proliferation in OVA‐immunized mice especially at a dose of 25 μg ( P <0.05, P <0.01, or P <0.001). The OVA‐specific serum IgG, IgG1, and IgG2b antibody levels were also significantly enhanced by 1 at a dose of 25 μg compared to the OVA control group ( P <0.05 or P <0.01). Moreover, the enhancing effect of 1 on the OVA‐specific IgG2b antibody responses to OVA in mice was more significant than that of Alum (Al(OH) 3 gel; P <0.01). These results suggest that 1 could be safely used as adjuvant with low or non‐haemolytic effect.