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A Water‐Soluble, Octacationic Zinc Phthalocyanine as Molecular Probe for Nucleic Acid Secondary Structure
Author(s) -
Zhang AnMing,
Huang Jing,
Weng XiaoCheng,
Li JinXi,
Ren LiGe,
Song ZhiBin,
Xiong XiaoQin,
Zhou Xiang,
Cao XiaoPing,
Zhou Yan
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790026
Subject(s) - chemistry , singlet oxygen , zinc , guanine , phthalocyanine , oligonucleotide , dna , titration , nucleic acid , fluorescence , cleavage (geology) , gel electrophoresis , photochemistry , oxygen , biochemistry , inorganic chemistry , organic chemistry , nucleotide , physics , geotechnical engineering , quantum mechanics , fracture (geology) , engineering , gene
The interaction between CT‐DNA and the zinc phthalocyanine ZnPc ( 1 ) was studied by UV/VIS and fluorescence titration, as well as by thermal denaturation. ZnPc was found to strongly bind to CT‐DNA ( K app =7.35×10 5 M −1 ) in a non‐intercalative mode. The photosensitized cleavage of pBR322 DNA was found to efficiently proceed via singlet‐oxygen ( 1 O 2 ) production. Further, ZnPc ( 1 ) caused site‐specific scission of guanine (G) bases around the bulge of the hairpin oligonucleotides OD1 – OD3 , as clearly shown by gel‐electrophoresis experiments.