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Preparation and PCR‐Amplification Properties of a Novel Amphiphilic Poly( N ‐vinylpyrrolidone) (PVP) Copolymer
Author(s) -
Zhang Lifen,
Liang Yong,
Meng Lingzhi,
Lu Xiaoju,
Liu Yunhai
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790021
Subject(s) - copolymer , chemistry , amphiphile , biocompatibility , micelle , aqueous solution , nuclear chemistry , poloxamer , critical micelle concentration , polymer , polymer chemistry , fluorescence , organic chemistry , physics , quantum mechanics
A novel amphiphilic copolymer, P(NVP‐ co ‐TrpAMT) ( 9 ) was prepared, comprising hydrophilic N ‐vinylpyrrolidone (NVP; 8 ) and hydrophobic ‘ N ‐[( tert ‐butoxy)carbonyl]tryptophanamido‐ N ′‐methacryl thiourea’ (TrpAMT; 7 ) segments. The amphiphilic copolymer 9 was characterized by 1 H‐NMR, GPC‐MALLS, TEM, and MTT assay. It has a critical micelle concentration ( cmc ) of 45.7 mg/l in aqueous solution, and good biocompatibility in vivo. According to TEM, the polymer is mostly present as spherical micelles in water, with a diameter of ca. 60–90 nm. In the presence of 0.1 μg/ml of 9 , the PCR amplification of the GC‐rich β ‐actin was efficiently enhanced. Also, the fluorescence intensity of the reporter dye SYBR Green I was increased by 26% at the 14th cycle during real‐time PCR of plasmid pUC18 DNA.