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Synthesis and Biological Studies of N ‐Alkylated Cyclic Diamines
Author(s) -
Xiong XiaoQin,
Liang Feng,
Yang Li,
Zhou Xiang,
Zheng CongYi,
Cao XiaoPing
Publication year - 2007
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200790005
Subject(s) - ethidium bromide , chemistry , hela , alkylation , dna , cleavage (geology) , in vitro , alkyl , quenching (fluorescence) , ligation , fluorescence , organic chemistry , biochemistry , microbiology and biotechnology , physics , geotechnical engineering , quantum mechanics , fracture (geology) , engineering , biology , catalysis
Several alkyl‐substituted mesocyclic diamines were synthesized, and their interaction with DNA were studied by melting‐temperature measurements and the ethidium bromide (EB)‐fluorescence competitive method. The supercoiled DNA hydrolytic cleavage by 1,4‐dioctyl‐1,4‐diazepan‐6‐ol ( 4 ) was supported by the evidence from free‐radical quenching and T4‐ligase ligation. Preliminary pharmacological tests showed that only 1,4‐dioctyl‐1,4‐diazepan‐6‐ol ( 4 ) had antitumor activity against HeLa cell lines in vitro.