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DNA‐Binding Studies of Mixed‐Ligand (Ethylenediamine)ruthenium(II) Complexes
Author(s) -
Nagababu Penumaka,
Latha J. Naveena Lavanya,
Satyanarayana Sirasani
Publication year - 2006
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200690123
Subject(s) - chemistry , intercalation (chemistry) , ruthenium , dna , ethylenediamine , stereochemistry , ligand (biochemistry) , phenanthroline , crystallography , receptor , inorganic chemistry , biochemistry , catalysis
A series of mixed‐ligand ruthenium(II) complexes of the type [Ru(en) 2 bpy] 2+ (bpy=2,2‐bipyridine; 1 ), [Ru(en) 2 phen] 2+ (phen=1,10‐phenantroline; 2 ), [Ru(en) 2 IP] 2+ (IP=imidazo[4,5‐ f ][1,10]phenanthroline; 3 ), and [Ru(en) 2 PIP] 2+ (PIP=2‐phenylimidazo[4,5‐ f ][1,10]phenanthroline; 4 ) have been isolated and characterized by UV/VIS, IR, and 1 H‐NMR spectral methods. The binding of the complexes with calf thymus DNA has been investigated by absorption, emission spectroscopy, viscosity measurements, DNA melting, and DNA photo‐cleavage. The spectroscopic studies together with viscosity measurements and DNA melting studies support that complexes 1 and 2 bind to CT DNA (=calf thymus DNA) by groove mode. Complex 2 binds more avidly to CT DNA than complex 1 , complexes 3 and 4 bind to CT DNA by intercalation mode, 4 binds more avidly to CT DNA than 3 . Noticeably, the four complexes have been found to be efficient photosensitisers for strand scissions in plasmid DNA.