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Investigation of de novo Totally Random Biosequences, Part III
Author(s) -
De Lucrezia Davide,
Franchi Marco,
Chiarabelli Cristiano,
Gallori Enzo,
Luisi Pier Luigi
Publication year - 2006
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200690089
Subject(s) - rna , folding (dsp implementation) , chemistry , denaturation (fissile materials) , chemical stability , nucleic acid structure , function (biology) , computational biology , biophysics , biochemistry , biology , microbiology and biotechnology , gene , organic chemistry , nuclear chemistry , electrical engineering , engineering
Fold is essential to RNA properties, and, in particular, its thermodynamic stability can be used to monitor RNA–protein or RNA–ligand interactions, and to engineer RNA with novel or improved properties. While clearly valuable, experimental determination of RNA folding stability by traditional biophysical techniques requires substantial amounts of pure sample and rather expensive equipment. In this paper, we report a new, simple approach to the determination of RNA folding stability by coupling enzymatic digestion and temperature denaturation. The assay, named RNA folding stability Test (RNA Foster), is designed to probe the fraction of folded RNA ( f fold ) in an equilibrium mixture of folded and unfolded ones as a function of temperature. The simplicity of RNA Foster suggests that it can easily be scaled up for high‐throughput studies of RNA folding stability both in basic and applied research.