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Photocytotoxic Pheophorbide‐Related Compounds from Aglaonema simplex
Author(s) -
Chee ChinFei,
Lee Hong Boon,
Ong Hean Chooi,
Ho Anthony SiongHock
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590134
Subject(s) - pheophorbide a , chemistry , ic50 , mtt assay , photodynamic therapy , stereochemistry , in vitro , biochemistry , organic chemistry
In our screening program for new photosensitizers from the Malaysian biodiversity, we found five pheophorbide‐related compounds from the leaves and stems of Aglaonema simplex. Detailed spectroscopic analyses showed that compounds 1 – 3 and 5 are pheophorbide and hydroxy pheophorbide derivatives of chlorophyll a and b. Compound 4 , identified as 15 1 ‐hydroxypurpurin‐7‐lactone ethyl methyl diester, was isolated for the first time from the Araceae family. An MTT‐based short‐term survival assay showed that all five compounds exhibit moderate‐to‐strong photocytotoxic activities towards human leukemia (HL60) and two oral squamous carcinoma cell lines (HSC‐2 and HSC‐3). Compounds 4 and 5 showed the strongest photocytotoxicities, with IC 50 values of 0.30–0.41 μ M ( Table 2 ). Compounds 1 – 3 with Et chains at C(17 3 ) were less photocytotoxic than the parent pheophorbide a ( 5 ).