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Cytotoxic Activity and Constituents of the Volatile Oil from the Roots of Patrinia scabra B unge
Author(s) -
Sun Hongxiang,
Sun Cuirong,
Pan Yuanjian
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590107
Subject(s) - chemistry , cytotoxicity , steam distillation , cytotoxic t cell , traditional medicine , chromatography , essential oil , in vitro , biochemistry , medicine
The volatile oil from the roots of Patrinia scabra B unge was isolated by steam distillation, and separated into four major fractions ( Fr. A–D ) by means of column chromatography. A total of 39 compounds ( 1 – 39 ) were identified by GC/MS analysis, and evaluated for their in vitro cytotoxic activities against human ovarian carcinoma cells (HO‐8910) and human hepatoma cells (Bel‐7402) ( Table 1 ). Fr. A showed the strongest inhibitory effect on HO‐8910 ( IC 50 =21 μg/ml) and Bel‐7402 cells (16 μg/ml), whereas Fr. B was the least active (>100 μg/ml). By comparison of the constituents of the four fractions, we assume that the cytotoxicity of the volatile oil of P. scabra is mainly due to the lignans and azulenes, rather than to caryophyllene oxide I ( 18 ). Our results suggest that the volatile oil of P. scabra possesses potent and tumor‐specific cytotoxicity, and could serve as a possible candidate for future cancer chemotherapy.