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Novel Peptide‐Based Copper(II) Complexes for Total Hydrolytic Cleavage of DNA
Author(s) -
Reddy Pulimamidi R.,
Manjula Pallerla,
Mohan Sepuru K.
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590106
Subject(s) - chemistry , phosphodiester bond , cleavage (geology) , copper , potentiometric titration , dna , peptide , cleave , circular dichroism , histidine , stereochemistry , crystallography , amino acid , organic chemistry , biochemistry , ion , rna , geotechnical engineering , fracture (geology) , engineering , gene
Stable Cu II complexes with histamine‐ and histidine‐containing dipeptides histidylserine and histidylphenylalanine have been developed. Their interaction in solution has been investigated, and the stability of their complexes was determined. The nature of binding in these complexes has been explained with the help of potentiometric pH titrations and 1 H‐NMR spectroscopy. The geometry of these complexes has been established by electronic spectra. The DNA‐binding and ‐cleavage abilities of these Cu II complexes have been probed by the absorption, thermal denaturation, fluorescence, and electrophoresis experiments. The results suggest that these peptide‐based Cu II complexes effectively bind and efficiently cleave DNA under mild biological conditions. Since Cu II complexes are known to play an important role in phosphodiester bond cleavages, these results assume importance.