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Ternary Zinc(II)–Dipeptide Complexes for the Hydrolytic Cleavage of DNA at Physiological pH
Author(s) -
Reddy Pulimamidi R.,
Mohan Sepuru K.,
Rao Kandibanda S.
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590043
Subject(s) - chemistry , intercalation (chemistry) , ternary complex , titration , dna , ternary operation , zinc , dipeptide , hydrolysis , fluorescence spectroscopy , cleavage (geology) , cleave , fluorescence , dna supercoil , stereochemistry , inorganic chemistry , crystallography , enzyme , organic chemistry , biochemistry , amino acid , dna replication , physics , geotechnical engineering , quantum mechanics , fracture (geology) , computer science , programming language , engineering
A series of Zn II complexes with cysteinylglycine (CysGly) and histidylserine (HisSer), and of CysGly and histidylphenylalanine (HisPhe) were investigated. Complex stabilities were determined potentiometrically, and binding geometries were probed by means of 1 H‐NMR spectroscopy, using Co II instead of Zn II as a spectroscopic marker. The ternary 1 : 1 : 1 complexes [Zn II (CysGly)(HisSer)] and [Zn II (CysGly)(HisPhe)] were shown by UV experiments, fluorescence titration, and gel electrophoresis to intercalate with DNA, and to hydrolytically cleave supercoiled DNA (form‐I), partly also circular (form‐II) DNA, under physiological conditions (37°, H 2 O, pH 7.5).