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Formation of a New Oxidative Metabolite from Kobophenol A by Human Intestinal Bacterium Klebsiella pneumoniae
Author(s) -
Liang Gaolin,
Qian Lisheng,
Cui Yiling,
Hu Changqi
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590031
Subject(s) - metabolite , chemistry , oxidative phosphorylation , metabolic pathway , klebsiella pneumoniae , feces , mass spectrometry , metabolism , high performance liquid chromatography , human feces , chromatography , in vitro , metabolomics , bacteria , biochemistry , microbiology and biotechnology , escherichia coli , biology , genetics , gene
During our studies on the metabolism of kobophenol A ( 1 ) in rats, we had isolated, purified, and identified the main new oxidative metabolite of 1 , koboquinone A ( 2 ) from rats' feces. To elucidate the metabolic pathway of 1 in rats, we conducted the in vitro metabolic experiments of 1 by human intestinal bacteria and found that Klebsiella pneumoniae produced appreciable amounts of 2 . This was verified by means of high performance liquid chromatography mass/mass spectrometry (HPLC/MS/MS) analysis.