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Structure Elucidation and Phytotoxicity of Ecdysteroids from Chenopodium album
Author(s) -
DellaGreca Marina,
D'Abrosca Brigida,
Fiorentino Antonio,
Previtera Lucio,
Zarrelli Armando
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200590025
Subject(s) - chemistry , germination , lactuca , elongation , chromatography , phytotoxicity , acetone , chenopodium , centrifugation , silica gel , brassica , shoot , botany , biochemistry , biology , materials science , metallurgy , weed , ultimate tensile strength
The leaves of Chenopodium album were infused in H 2 O/MeOH. The extract treated with cold acetone gave heavy precipitation, which was removed by centrifugation. Solid material was fractionated into acidic and neutral fractions. The acidic material was subjected to different silica‐gel column chromatographies, and then it was purified by reversed‐phase HPLC to afford four known ecdysteroids and the new 3 β ,14 α ‐dihydroxy‐5 β ‐pregn‐7‐ene‐2,6,20‐trione that were characterized by extensive spectroscopic investigation, especially by 1D‐ and 2D‐NMR. Their effects on germination and growth of Lactuca sativa L. have been studied. The results are reported as percentage differences of germination, root elongation and shoot elongation, from the control at concentrations ranging from 10 −4 to 10 −7 M .