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Isolation and Lipoxygenase‐Inhibition Studies of Phenolic Constituents from Ehretia obtusifolia
Author(s) -
Iqbal Kiran,
Nawaz Sarfraz Ahmad,
Malik Abdul,
Riaz Naheed,
Mukhtar Naveen,
Mohammad Pir,
Choudhary M. Iqbal
Publication year - 2005
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200490161
Subject(s) - chemistry , lipoxygenase , isolation (microbiology) , traditional medicine , biochemistry , enzyme , microbiology and biotechnology , biology , medicine
The phenolic compounds methyl 2‐ O ‐feruloyl‐1a‐ O ‐vanillactate ( 1 ), caffeic anhydride ( 2 ), and trans 4‐hydroxycyclohexyl‐2‐ O‐p ‐coumaroyl β ‐ D ‐glucopyranoside ( 3 ) have been isolated from the AcOEt‐soluble fraction of Ehretia obtusifolia , along with methyl rosmarinate ( 4 ) and rosmarinic acid ( 5 ), which are reported for the first time from this species. Their structures were determined by means of 1D‐ and 2D‐NMR techniques. Compounds 1 – 5 inhibited lipoxygenase in a concentration‐dependent manner, with K i values ranging from 0.85–57.6 μ M . Compounds 1, 2, 4 , and 5 showed noncompetitive inhibition, whereas 3 was found to be an uncompetitive inhibitor of lipoxygenase.