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Synthesis and Applications of OligonucleotideCarbohydrate Conjugates
Author(s) -
Zatsepin Timofei S.,
Oretskaya Tatiana S.
Publication year - 2004
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200490104
Subject(s) - oligonucleotide , chemistry , nucleoside , carbohydrate , conjugate , biochemistry , dna , solid phase synthesis , conjugated system , combinatorial chemistry , polymer , organic chemistry , peptide , mathematical analysis , mathematics
Nowadays, oligonucleotidecarbohydrate conjugates are used in antisense biotechnology and in the study of glycosylated DNA functioning in vitro. The application of mono‐ and disaccharide phosphoramidites, solid‐phase supports with immobilized carbohydrates, glycosylated nucleoside phosphoramidites, and postsynthetic conjugation of reactive sugar derivatives with oligonucleotides for preparation of oligonucleotidecarbohydrate conjugates have been systematically studied. The advantages and disadvantages of these approaches are considered. Possible strategies for synthesis of glycoclusters with different topologies conjugated to DNA are discussed. Applications of oligonucleotidecarbohydrate conjugates are highlighted. Studies of interactions of glycosylated oligonucleotides with proteins and effective cell‐specific delivery of oligonucleotidecarbohydrate conjugates are discussed.