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Resolution of Binary Mixtures of Rifamycin SV and Rifampicin by UV/VIS Spectroscopy and Partial Least‐Squares Method (PLS)
Author(s) -
Lomillo M. Asunción Alonso,
Domínguez Renedo Olga,
Arcos Martínez M. Julia
Publication year - 2004
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200490096
Subject(s) - partial least squares regression , chemistry , rifampicin , resolution (logic) , analytical chemistry (journal) , chromatography , linear regression , spectroscopy , ultraviolet visible spectroscopy , binary number , biological system , statistics , mathematics , artificial intelligence , computer science , antibiotics , physics , biochemistry , arithmetic , quantum mechanics , biology , organic chemistry
A procedure is proposed for the joint determination of rifamycin SV and rifampicin by UV/VIS spectroscopy. A partial least‐squares regression (PLS) was used for the resolution of the overlapping spectrophotometric signals from mixtures of the two drugs. The application of a genetic algorithm to select some of the predictor variables allows one to considerably reduce the number of experimental variables, as well as to improve the prediction capacity of the PLS model constructed with these selected potentials. Finally, the methods were applied to the determination of these drugs in biological samples.