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Synthesis of OligonucleotidePeptide Conjugates Carrying the c‐ myc Peptide Epitope as Recognition System
Author(s) -
Frieden Miriam,
Aviñó Anna,
Tarrasón Gema,
Escorihuela Marta,
Piulats Jaume,
Eritja Ramon
Publication year - 2004
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200490075
Subject(s) - oligonucleotide , chemistry , peptide , conjugate , epitope , monoclonal antibody , nucleoside , biochemistry , combinatorial chemistry , microbiology and biotechnology , dna , antibody , biology , mathematical analysis , mathematics , immunology
Oligonucleotidepeptide conjugates 1 – 3 were prepared by sequential addition of the appropriate Boc‐protected amino acids, followed by nucleoside phosphoramidites in the same support. These molecules are designed to be used for triplex formation and for the directed assembly of nanomaterials. The structures of the desired oligonucleotidepeptide conjugates were confirmed by mass spectrometry on small oligonucleotidepeptide conjugates, by gel electrophoresis, and by hybridization with complementary oligonucleotides. Oligonucleotides carrying the c‐ myc peptide were specifically recognized by the anti‐c‐ myc monoclonal antibody.