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Enantioselective Synthesis of ( S )‐3‐Carboxy‐4‐((carboxy)difluoromethyl)phenylalanine in Protected Form and Its Incorporation into a PTP‐1B‐Directed Tripeptide
Author(s) -
Kang Sang Uk,
Gao Yang,
Wu Li,
Zhang ZhongYin,
Burke Terrence R.
Publication year - 2004
Publication title -
chemistry and biodiversity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 70
eISSN - 1612-1880
pISSN - 1612-1872
DOI - 10.1002/cbdv.200490053
Subject(s) - tripeptide , chemistry , stereochemistry , phenylalanine , peptide , oligopeptide , amino acid , enantioselective synthesis , in vitro , amide , peptide synthesis , combinatorial chemistry , biochemistry , catalysis
Recent findings have shown that, when expressed in the tripeptide platform, ‘Fmoc‐Glu‐Xxx‐Leu‐amide’, the phosphotyrosyl mimetic (pTyr), Xxx=( S )‐3‐carboxy‐4‐(carboxymethyl)‐Phe, provides higher PTP‐1B affinity than that obtained with Xxx=( S )‐difluorophosphonomethyl‐Phe (F 2 Pmp). This was of note, since difluorophosphonomethyl‐containing pTyr mimetics have typically exhibited higher PTP‐inhibitory potencies than carboxy‐based mimetics, indicating the potential value of 3‐carboxy‐4‐(carboxymethyl)‐Phe as a starting point for further analogue development. Therefore, relying on precedence that α ‐fluorination often enhances PTP‐1B affinity, ( S )‐3‐carboxy‐4‐((carboxy)difluoromethyl)‐Phe was designed as a PTP‐1B‐directed pTyr mimetic. Reported herein is the synthesis of this new amino acid analogue through application of commercially available Williams chiral auxiliary. The target was prepared in an orthogonally protected form suitable for peptide synthesis according to Fmoc chemistries and utilization for the synthesis of a PTP‐1B‐directed tripeptide bearing the sequence indicated above. Biological evaluation with in vitro PTP‐1B assays indicated nearly total loss of affinity for this peptide. Evidence is provided that the unexpected deleterious effect of fluorination is probably not related to p K a effects.