Open AccessClinical significance and functional validation of PPA1 in various tumors
Author(s) -
Luo Dehong,
Wang Guanwen,
Shen Wenzhi,
Zhao Shuangtao,
Zhou Wei,
Wan Lin,
Yuan Liying,
Yang Shuang,
Xiang Rong
Publication year - 2016
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.894
Subject(s) - carcinogenesis , immunohistochemistry , ovarian tumor , ovarian cancer , lung cancer , cell growth , cancer research , apoptosis , biology , western blot , pathological , cancer , pathology , cell , medicine , gene , biochemistry , genetics
Abstract The aim of the study was to detect PPA 1 expression in various tumors and to investigate the relationship between PPA 1 expression and clinicopathological parameters to further analyze its clinical significance. Immunohistochemical staining detected PPA 1 expression in 305 noncancerous tissues and 675 tumor tissues, which included 12 different tumor types. QPCR and western blot examined PPA 1 expression in tumor‐derived cell lines including those derived from liver, breast, lung, and ovarian cancers. Cell proliferation and apoptosis assays were used to investigate PPA 1‐regulated cell growth in tumor cells. Finally, a bioinformatics analysis was used to verify the role of PPA 1 in carcinogenesis. Among the 12 types of tumors, PPA 1 expression was significantly higher in lung and ovarian cancers ( P < 0.001). In lung cancer, PPA 1 expression was associated with tumor size, patients’ age, and smoking status, whereas in ovarian cancer, PPA 1 expression was associated with pathological grade ( P < 0.05). Moreover, we found that PPA 1 expression was up‐regulated in lung and ovarian cancer cell lines compared with nontumor cells. In addition, suppression of PPA 1 expression by RNA interference in lung and ovarian cancer cells showed increased cell apoptosis and decreased cell proliferation, which was mediated by TP 53 and p21 signaling. Notably, a bioinformatics analysis was used to verify the function of PPA 1 in the development and progression of tumors. PPA 1 expression is significantly higher in many tumors, especially those of lung and ovarian origin, which suggests that PPA 1 plays an important role in carcinogenesis and in the development of some tumors.