Open AccessThe E3 ubiquitin ligase RNF135 regulates the tumorigenesis activity of tongue cancer SCC25 cells
Author(s) -
Jin Jian,
Zhao liya,
Li Zubing
Publication year - 2016
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.832
Subject(s) - ubiquitin ligase , carcinogenesis , ubiquitin , cancer research , viability assay , dna ligase , cancer cell , gene knockdown , in vivo , microbiology and biotechnology , cancer , biology , cell growth , cell , chemistry , apoptosis , biochemistry , genetics , enzyme , gene
Abstract Several E3 ubiquitin ligases have been confirmed that they are related to the tumorigenesis. This study aims to find the tongue cancer‐related E3 ubiquitin ligase. The E3 ubiquitin ligase library was screened. The effect of candidate molecule on tongue cancer was validated through cell viability, cell proliferation, colony formation, invasive assay in vitro, and the xenograft model in vivo. The E3 ubiquitin ligase RNF 135 significantly promoted the expression of PTEN and TP 53 in SCC 25 cells. The overexpression of RNF 135 inhibited the viability, proliferation, and invasion of SCC 25 cells. Knockdown of RNF 135 had the opposite effects. Furthermore, RNF 135 regulates the tumorigenesis activity of SCC 25 cells in vivo. Our results demonstrated that RNF 135 had the potential to affect the development of the tongue cancer in vitro. The further in vivo study is helpful to fully understand the role of it.