Open AccessThe long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
Author(s) -
Pan Zhiwen,
Mao Weimin,
Bao Yejiang,
Zhang Min,
Su Xinhua,
Xu Xiaohong
Publication year - 2016
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.770
Subject(s) - gene knockdown , downregulation and upregulation , long non coding rna , rna , cancer research , in vitro , cell migration , biology , cell , microbiology and biotechnology , esophageal squamous cell carcinoma , cell culture , cancer , gene , genetics
Abstract The objective of the study was to investigate the expression and functions of CASC 9 in esophageal squamous cell carcinoma ( ESCC ). Long noncoding RNA s (lnc RNA s) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC 9 was determined from clinical samples and cell lines by qRT ‐ PCR . The effects of CASC 9 knockdown on migration and invasion were evaluated by wound healing assay, cell migration and invasion assays in vitro. We found that the lnc RNA , CASC 9, was markedly upregulated in ESCC tissues. Furthermore, knockdown of CASC 9 significantly suppressed cell migration and invasion in vitro. Furthermore, enhanced CASC 9 expression level was correlated with differentiation. The results indicated that CASC 9 is significantly upregulated in ESCC tissues and may represent a new marker of poor prognosis and a potential therapeutic target for esophageal cancer intervention.