Open AccessHypoxia‐responsive miR‐124 and miR‐144 reduce hypoxia‐induced autophagy and enhance radiosensitivity of prostate cancer cells via suppressing PIM 1
Author(s) -
Gu Hao,
Liu Mingzhu,
Ding Changmao,
Wang Xin,
Wang Rui,
Wu Xinyu,
Fan Ruitai
Publication year - 2016
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.664
Subject(s) - radioresistance , downregulation and upregulation , prostate cancer , autophagy , hypoxia (environmental) , cancer research , radiosensitivity , microrna , dicer , cancer cell , cancer , biology , chemistry , microbiology and biotechnology , cell culture , apoptosis , medicine , small interfering rna , transfection , radiation therapy , gene , biochemistry , genetics , organic chemistry , oxygen
Cancer cells in hypoxia usually make adaptive changes in cellular metabolism, such as altered autophagy. This might be a cause of enhanced radioresistance in some types of cancer. In this study, we investigated hypoxia‐responsive mi RNA s in two prostate cancer cell lines ( DU 145 and PC 3). This study firstly reported that hypoxia induces further downregulation of miR‐124 and miR‐144, which might be a result of impaired dicer expression. These two mi RNA s can simultaneously target 3′ UTR of PIM 1. Functional study showed that miR‐124 or miR‐144 overexpression can inhibit hypoxia‐induced autophagy and enhance radiosensitivity at least via downregulating PIM 1. Therefore, hypoxia induced miR‐124 and miR‐144 downregulation may contribute to a prosurvival mechanism of prostate cancer cells to hypoxia and irradiation at least through attenuated suppressing of PIM 1. This finding presents a potential therapeutic target for prostate cancer.