Open AccessComparative study of 18 F‐ FDG ‐ PET / CT imaging and serum hTERT mRNA quantification in cancer diagnosis
Author(s) -
Ping Bingqiong,
Tsuno Satoshi,
Wang Xinhui,
Ishihara Yoshitaka,
Yamashita Taro,
Miura Keigo,
Miyoshi Fuminori,
Shinohara Yuki,
Matsuki Tsutomu,
Tanabe Yoshio,
Tanaka Noriaki,
Ogawa Toshihide,
Shiota Goshi,
Miura Norimasa
Publication year - 2015
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.508
Subject(s) - telomerase reverse transcriptase , medicine , cancer , metastasis , fluorodeoxyglucose , positron emission tomography , messenger rna , nuclear medicine , telomerase , chemistry , gene , biochemistry
Abstract We have reported on the clinical usefulness of human telomerase reverse transcriptase ( hTERT ) mRNA quantification in sera in patients with several cancers. Positron emission tomography–computed tomography ( PET / CT ) using 18 F‐fluorodeoxyglucose ( FDG ) has recently become an excellent modality for detecting cancer. We performed a diagnostic comparative study of FDG ‐ PET / CT and hTERT mRNA quantification in patients with cancer. Four hundred seventy subjects, including 125 healthy individuals and 345 outpatients with cancer who had received medical treatments for cancer in their own or other hospitals, were enrolled. The subjects were diagnosed by FDG ‐ PET / CT , and we measured their serum hTERT mRNA levels using real‐time RT ‐ PCR , correlating the quantified values with the clinical course. In this prospective study, we statistically assessed the sensitivity and specificity, and their clinical significance. hTERT mRNA and FDG ‐ PET / CT were demonstrated to be correlated with the clinical parameters of metastasis and recurrence ( P < 0.001), and of recurrence and tumor number in cancer compared with noncancer patients, respectively. A multivariate analysis showed a significant difference in the detection by FDG ‐ PET / CT , 18 F‐ FDG uptake, the detection by hTERT mRNA , and age. The use of both FDG ‐ PET / CT and hTERT mRNA resulted in a positivity of 94.4% (221/234) for the detection of viable tumor cells. FDG ‐ PET / CT is superior to hTERT mRNA quantification in the early detection of cancer and combinative use of FDG ‐ PET / CT and hTERT mRNA may improve the diagnostic accuracy of cancer.