Open AccessmiR‐106b‐5p promotes aggressive progression of hepatocellular carcinoma via targeting RUNX3
Author(s) -
Gu Hao,
Gu Shensen,
Zhang Xinlong,
Zhang Songjiang,
Zhang Dongming,
Lin Junsheng,
Hasengbayi Saiken,
Han Wei
Publication year - 2019
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.2511
Subject(s) - hepatocellular carcinoma , downregulation and upregulation , matrigel , microrna , cancer research , medicine , reporter gene , proportional hazards model , gene expression , oncology , gene , biology , angiogenesis , biochemistry
Background and Objectives The roles of microRNA(miR)‐106b‐5p in hepatocellular carcinoma (HCC) remain unclear. We aimed here to investigate the clinical significance of miR‐106b‐5p expression in HCC and its underlying mechanisms. Methods Expression levels of miR‐106b‐5p in 108 HCC clinical samples by quantitative real‐time reverse transcription PCR. Associations of miR‐106b‐5p expression with various clinicopathological features and patients' prognosis were evaluated by Chi‐square test, Kaplan‐Meier, and Cox proportional regression analyses, respectively. The target gene of miR‐106b‐5p and their functions in HCC cells were investigated by luciferase reporter, CCK‐8, and Transwell Matrigel invasion assays. Results miR‐106b‐5p expression was markedly higher in HCC tissues than in noncancerous adjacent liver tissues ( P < .001). miR‐106b‐5p upregulation was significantly associated with advanced TNM stage ( P = .02), short recurrence‐free ( P = .005), and overall ( P = .001) survivals. Importantly, miR‐106b‐5p expression was an independent predictor of poor prognosis ( P < .05). RUNX3 was identified as a direct target gene of miR‐106b‐5p in HCC cells. Functionally, miR‐106b‐5p upregulation promoted the viability and invasion of HCC cells, while enforced RUNX3 expression reversed the oncogenic effects of miR‐106b‐5p overexpression. Conclusions miR‐106b‐5p may serve as a potent prognostic marker for tumor recurrence and survival of HCC patients. miR‐106b‐5p may exert an oncogenic role in HCC via regulating its target gene RUNX3.