Open AccessHigh CD49f expression is associated with osteosarcoma tumor progression: a study using patient‐derived primary cell cultures
Author(s) -
Penfornis Patrice,
Cai David Z.,
Harris Michael R.,
Walker Ryan,
Licini David,
Fernandes Joseph D. A.,
Orr Griffin,
Koganti Tejaswi,
Hicks Chindo,
Induru Spandana,
Meyer Mark S.,
Khokha Rama,
Barr Jennifer,
Pochampally Radhika R.
Publication year - 2014
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.249
Subject(s) - cd90 , cancer research , mesenchymal stem cell , cell , cell growth , tumor progression , cancer stem cell , osteosarcoma , circulating tumor cell , cell culture , population , biology , stem cell , cancer , cd44 , medicine , microbiology and biotechnology , metastasis , genetics , environmental health
Overall prognosis for osteosarcoma ( OS ) is poor despite aggressive treatment options. Limited access to primary tumors, technical challenges in processing OS tissues, and the lack of well‐characterized primary cell cultures has hindered our ability to fully understand the properties of OS tumor initiation and progression. In this study, we have isolated and characterized cell cultures derived from four central high‐grade human OS samples. Furthermore, we used the cell cultures to study the role of CD49f in OS progression. Recent studies have implicated CD49f in stemness and multipotency of both cancer stem cells and mesenchymal stem cells. Therefore, we investigated the role of CD49f in osteosarcomagenesis. First, single cell suspensions of tumor biopsies were subcultured and characterized for cell surface marker expression. Next, we characterized the growth and differentiation properties, sensitivity to chemotherapy drugs, and anchorage‐independent growth. Xenograft assays showed that cell populations expressing CD49f hi /CD90 lo cell phenotype produced an aggressive tumor. Multiple lines of evidence demonstrated that inhibiting CD49f decreased the tumor‐forming ability. Furthermore, the CD49f hi /CD90 lo cell population is generating more aggressive OS tumor growth and indicating this cell surface marker could be a potential candidate for the isolation of an aggressive cell type in OS s.