Open AccessDifferences in gene expression between high‐grade dysplasia and invasive HPV + and HPV − tonsillar and base of tongue cancer
Author(s) -
Haeggblom Linnea,
ÄhrlundRichter Andreas,
Mirzaie Leila,
Farrajota Neves da Silva Pedro,
Ursu Ramona G.,
Ramqvist Torbjörn,
Näsman Anders
Publication year - 2019
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.2450
Subject(s) - dysplasia , cancer , hpv infection , cervical cancer , immunohistochemistry , cancer research , pathology , gene , carcinoma , medicine , biology , genetics
Abstract Background Human papillomavirus (HPV) is a causative agent for tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC), as well as for cervical cancer. Premalignant stages in cervical cancer have been studied extensively, while little is known about premalignant stages in TSCC/BOTSCC and the role of HPV. Here we analyzed differences in gene and protein expression between high‐grade dysplasia and invasive cancer in both HPV‐positive (HPV + ) and HPV‐negative (HPV − ) TSCC/BOTSCC. Methods High‐grade dysplasia and invasive carcinoma were laser microdissected from HPV + and HPV − TSCC/BOTSCC tumor sections. Differential gene expression was studied utilizing nanoString RNA‐panels and genes of interest were validated on the protein level by immunohistochemistry. Results Forty genes in the HPV + tumors showed significantly different expression between high‐grade dysplasia and invasive cancer and 33 genes in the HPV − tumors. Five out of the nine most significant pathways showed similar increased activity in invasive cancer as compared to high‐grade dysplasia in both HPV + and HPV − tumors. Lastly, significant differences in protein expression was confirmed for SPARC, psoriasin, type I collagen and galectin‐1 in both HPV + and HPV − tumors. Conclusions This is to our knowledge the first study disclosing differences and similarities in gene expression between dysplastic and invasive HPV + and HPV − TSCC/BOTSCC.