Open AccessThe human ATF 1 rs11169571 polymorphism associated with risk of nasopharyngeal carcinoma in Southern Chinese populations
Author(s) -
Peng Shutang,
Huang GuoLiang,
Xu Nansong,
Lu Yan,
Zeng Liuyan,
Li Xin,
Luo Shengqun,
Lyu Xiaoming,
Jiang Qiang,
Li Tong,
He Zhiwei
Publication year - 2019
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.2022
Subject(s) - nasopharyngeal carcinoma , allele , genotype , carcinogenesis , biology , microbiology and biotechnology , luciferase , risk factor , medicine , transcription factor , allele frequency , immunology , gene , cancer research , genetics , transfection , radiation therapy
Our previous work reported activating transcription factor 1 ( ATF 1) is a promotive factor of nasopharyngeal carcinoma ( NPC ) tumorigenesis. This study is to further explore the association between the human ATF 1 rs11169571 polymorphism and the risk of NPC occurrence. The association between ATF 1 rs11169571 and risk of NPC occurrence was investigated in clinical samples of 560 patients and 661 controls obtained from southern China with high incidence of NPC . The genotypes were detected by PCR ‐ RFLP . The differential expression activity of alleles ‐T and ‐C was analyzed with CNE ‐2 and C666‐1 cells by luciferase reporter assay. Our data suggested that the allelic frequency and genotypes were significantly different between patients and controls. Compared to the TT homozygote, the TC and CC genotypes have been shown to be significantly decreased in NPC patients ( OR = 0.494, 95% CI = 0.387‐0.629, P < 0.001 and OR = 0.556, 95% CI = 0.364‐0.851, P = 0.007, respectively). Compared to the ‐T allele, the ‐C allele is a factor of decreased risk in NPC ( OR = 0.642, 95% CI = 0.537‐0.767, P < 0.001). Luciferase reporter activity revealed that the ‐T allele confers a higher expression activity than the ‐C allele in CNE 2 cells and C666‐1 cells. In conclusion, ATF 1 rs11169571 which could affect the expression of ATF 1 is associated with NPC risk.