Open AccessLong noncoding RNA and mRNA profiling in cetuximab‐resistant colorectal cancer cells by RNA sequencing analysis
Author(s) -
Jing Changwen,
Ma Rong,
Cao Haixia,
Wang Zhuo,
Liu Siwen,
Chen Dan,
Wu Yang,
Zhang Junying,
Wu Jianzhong
Publication year - 2019
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.2004
Subject(s) - cetuximab , rna , rna interference , non coding rna , messenger rna , small interfering rna , biology , long non coding rna , microbiology and biotechnology , colorectal cancer , cancer research , downregulation and upregulation , microrna , cancer , gene , genetics
To gain an insight into the molecular mechanisms of cetuximab resistance in colorectal cancer, we generated a cetuximab‐resistant cell line (H508/ CR ) and performed RNA sequencing to identify the differential expression patterns of noncoding RNA s (nc RNA s) and mRNA s between cetuximab‐sensitive and resistant cells. A total of 278 nc RNA transcripts and 1,059 mRNA transcripts were dysregulated in the cetuximab‐resistant cells. The expression levels of nine selected long noncoding RNA s (lnc RNA s) were validated using quantitative real‐time PCR . Functional analysis revealed that several groups of lnc RNA s might be involved in pathways associated with cetuximab resistance. Increased glucose consumption and lactate secretion in cetuximab‐resistant cells suggested that glucose metabolism might be involved in cetuximab resistance. In addition, lnc RNA LINC 00973 was upregulated in the H508/ CR cell line and cells transfected with a LINC 00973 short interfering RNA exhibited reduced cell viability, increased apoptosis, and decreased glucose consumption and lactate secretion. Our results provide essential data regarding differentially expressed lnc RNA s and mRNA s in cetuximab‐resistant cells, which may provide new potential candidates for cetuximab therapy.