Dysregulated circular RNA s in medulloblastoma regulate proliferation and growth of tumor cells via host genes

Circular RNA s (circ RNA s) have been demonstrated to be involved in various biological processes. Nevertheless, the function of circ RNA s in medulloblastoma ( MB ) is still unknown. The present study aimed to investigate the expression profiles of circ RNA s and related mechanisms for regulating the proliferation and growth of tumor cells in MB . The expression profiles of circ RNA s were screened from four normal cerebellum and four MB samples using a HiSeq Sequencer. Bioinformatic analysis was employed to predict the interaction between circ RNA s and mRNA s in MB . Subsequently, the expression levels of eight differential circ RNA s [circ‐ SKA 3 (hsa_circ_0029696), circ‐ DTL (hsa_circ_0000179), circ‐ CRTAM , circ‐ MAP 3K5 (hsa_circ_0006856), circ‐ RIMS 1‐1 (hsa_circ_0132250), circ‐ RIMS 1‐2 (hsa_circ_0076967), circ‐ FLT 3‐1 (hsa_circ_0100165), and circ‐ FLT 3‐2 (hsa_circ_0100168)] were validated using quantitative reverse transcription−polymerase chain reaction. Moreover, circ‐ SKA 3 and circ‐ DTL were silenced using small interfering RNA s and their host genes were overexpressed to investigate their role in the pathogenesis of MB . A total of 33 circ RNA s were found to be differentially expressed in MB tissues (fold change ≥ 2.0, FDR <0.05), of which three were upregulated and 30 were downregulated; six circ RNA s were experimentally validated successfully. Upregulated circ‐ SKA 3 and circ‐ DTL promoted the proliferation migration and invasion in vitro by regulating the expression of host genes. This novel study exploited the profiling of circ RNA s in MB and demonstrated that circ‐ SKA 3 and circ‐ DTL were crucial in the tumorigenesis and development of MB and might be considered as novel and potential biomarkers for the diagnosis and new targets for the intervention of MB .