Distribution and role of high‐risk human papillomavirus genotypes in women with cervical intraepithelial neoplasia: A retrospective analysis from Wenzhou, southeast China

To add the growing literature on baseline of high‐risk human papillomavirus ( HR ‐ HPV ) genotype distribution in cervical intraepithelial neoplasia ( CIN ) before the widespread using of HPV vaccines in Chinese mainland and to improve risk stratification of HR ‐ HPV –positive women. Retrospectively, the data of age, cervical HPV genotypes, cytology, and pathology were collected from 1166 patients who received loop electrosurgical excision procedure ( LEEP ). HPV genotypes were analyzed with Flowcytometry Fluorescence Hybridization Method. And then HPV prevalence, HR ‐ HPV genotype distribution and the correlation of HR ‐ HPV genotypes with CIN 2+ ( CIN 2 or severer) were analyzed. The role of multiple HR ‐ HPV types infection with or without HPV 16/18 in the pathogenesis of CIN 2+ was also analyzed. The 6 most common HR ‐ HPV genotypes were HPV 16, 58, 52, 33, 18, and 31 in descending order. Compared to HR ‐ HPV –negative women, HPV 16, 33 or 58 positive women had higher risk of CIN 2+ ( OR  = 5.10, 95% CI  = 2.68‐9.70; OR  = 3.09, 95% CI  = 1.39‐6.84; OR  = 3.57, 95% CI  = 1.85‐6.89, respectively). And women who were infected by multiple HR ‐ HPV types infection with HPV 16/18 also had higher risk of CIN 2+ ( OR  = 2.58, 95% CI  = 1.35‐4.92). However, multiple HR ‐ HPV types infection without HPV 16/18 did not increase the risk significantly ( P  = .08). Compare to bivalent Cervarix ® and quadrivalent Gardasil ® , HPV prophylactic vaccine targeting HPV 31, 33, 52, and 58 might provide women more protection from HPV ‐induced cervical cancer in China. The women who infected by HPV 16, 33, 58, or multiple HR ‐ HPV types with HPV 16/18 have higher risk of CIN 2+ and need to be paid more attention in screening processes. And the role of multiple HR ‐ HPV types infection without HPV 16/18 needs be further identified in more studies.