Open AccessMi RNA ‐545 negatively regulates the oncogenic activity of EMS 1 in gastric cancer
Author(s) -
Ma Min,
Zhao Juanxia,
Wu Qunfeng,
Xiao Ke,
Li Shuang,
Zhu Haizhen,
Liu Chen,
Xie Hailong,
Zuo Chaohui
Publication year - 2018
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1520
Subject(s) - microrna , messenger rna , oligonucleotide , cancer research , cancer , metastasis , biology , epithelial–mesenchymal transition , gastric carcinoma , microbiology and biotechnology , gene , chemistry , biochemistry , genetics
Gastric cancer ( GC ) is a common malignant tumor of the digestive system. In addition, GC metastasis is an extremely complicated process. In this article, high expression levels of EMS 1 mRNA and protein were found to be positively correlated with an enhanced malignant potential of GC cells and a poor clinical prognosis of GC patients. Interestingly, the expression levels of EMS 1 mRNA and protein in GC cells were inhibited by micro RNA ‐545 (miR‐545), which was identified by a bioinformatics analysis. The expression level of miR‐545 in carcinoma tissues was significantly lower than that in para‐carcinoma tissues. The proliferation and epithelial‐mesenchymal transition ( EMT ) of GC cells were suppressed by exogenous oligonucleotides of miR‐545 mimics. In addition, the expression levels of EMT ‐associated markers were altered with the expression of miR‐545. Notably, the growth rates of tumors in nude mice were seriously restrained by an intratumoral injection of oligonucleotides of the miR‐545 mimics. These results suggest a negative regulatory role of miR‐545 on the oncogenic activity of EMS 1. In addition, EMS 1 and miR‐545 may be potential biomarkers for GC diagnosis. Synthesized oligonucleotides of miR‐545 mimics may be developed as important gene medicines for GC therapy in the future.