Open AccessmiR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group AT ‐hook 2
Author(s) -
Xu Li,
Du Bin,
Lu QiJue,
Fan XiaoWen,
Tang Ke,
Yang Lie,
Liao WeiLin
Publication year - 2018
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1491
Subject(s) - hmga2 , cell growth , microrna , cancer research , carcinogenesis , cell , cell culture , blot , immunocytochemistry , biology , lung cancer , cell migration , cancer , pathology , medicine , gene , biochemistry , genetics , endocrinology
An increasing number of studies have demonstrated that micro‐ribonucleic acids (mi RNA s) are important tumor suppressors during carcinogenesis. However, the function of mi RNA ‐541 (miR‐541) in malignancies, especially lung cancer, has not been widely reported. In this study, miR‐541 expression was significantly decreased in squamous cell lung carcinoma ( SCLC ) cancerous tissue and SCLC cell lines. To analyze miR‐541 function in SCLC , we overexpressed miR‐541 in SCLC cell lines ( SK ‐ MES ‐1 and H226). According to the CCK 8, wound scratch, and transwell invasion assay results, miR‐541 overexpression significantly inhibited SCLC cell proliferation, migration, and invasion ability. Next, using RT ‐ PCR , Western blotting, immunocytochemistry, and luciferase assays, HMGA 2 was identified, for the first time, as a direct regulatory target of miR‐541 in SK ‐ MES ‐1 and H226 cells. Furthermore, upregulating HMGA 2 expression significantly alleviated the suppressive effects of miR‐541 on SK ‐ MES ‐1 and H226 cell proliferation, migration, and invasion. In summary, our study revealed that miR‐541 inhibited SCLC proliferation and invasion by directly targeting HMGA 2.