Open AccessEstablishment and characterization of a novel lymphangiosarcoma cell line ( MO ‐ LAS ) compared with the hemangiosarcoma cell line ( ISO ‐ HAS )
Author(s) -
Masuzawa Mikio,
Masuzawa Mamiko,
Hamada Yuhko,
Arakawa Nobuko,
Mori Mari,
Ishii Masako,
Nishiyama Shigeo
Publication year - 2012
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.12
Subject(s) - angiosarcoma , hemangiosarcoma , cell culture , medicine , cancer research , pathology , biology , genetics
The concept of “lymphangiosarcoma” remains obscure. Therefore, we reported a patient with lymphangiosarcoma, resistant to immunotherapy. The patient presented with impressive and discriminative features: clinically an ill‐defined edematous lesion with lymphorrhea and pathologically atypical vascular channel formation without extravasation of blood, clearly distinguished from common angiosarcoma with hemorrhage. From this case, a lymphangiosarcoma cell line, MO ‐ LAS , was established and its characteristics were compared with the hemangiosarcoma cell line, ISO ‐ HAS . Flow cytometric analysis revealed that MO ‐ LAS was negative for factor VIII ‐related antigen, but positive for CD 31, D2‐40, NZ ‐1, and vascular endothelial growth factor receptor‐3 ( VEGFR ‐3), similar to ISO ‐ HAS . However, MO ‐ LAS expressed a much higher level of homeobox gene PROX1 , indicating a lymphatic phenotype, compared with ISO ‐ HAS . Furthermore, MO ‐ LAS showed a much lesser expression of oncogenes and much lower sensitivity against lymphokine‐activated killer ( LAK ) cells. Lymphangiosarcoma may be difficult to recognize by the immune system. Conclusively, the establishment of MO ‐ LAS , a novel angiosarcoma cell line bearing lymphatic characters, strongly suggests the entity of lymphangiosarcoma.