Open AccessA comprehensive genome‐wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma
Author(s) -
Cui Hongxia,
Zhang Yunxing,
Zhang Qiujie,
Chen Wenming,
Zhao Haibo,
Liang Jun
Publication year - 2017
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1180
Subject(s) - long non coding rna , rna , hepatocellular carcinoma , biology , metastasis , gene knockdown , cancer research , non coding rna , microarray analysis techniques , gene , gene expression , microarray , computational biology , genetics , cancer
Hepatocellular carcinoma ( HCC ) is one of the most common malignancies worldwide, especially in East Asia and China. Long noncoding RNA s (lnc RNA s) are emerging as critical regulators that may be involved in the development and progression of cancers in humans. However, the contributions of lnc RNA s to HCC development, metastasis, and recurrence remain largely unknown. In this study, we comprehensively investigated lnc RNA expression profile in HCC and normal tissues using TCGA RNA sequencing data, one RNA sequencing dataset, and two microarray datasets from GEO . By analyzing these four datasets, we identified hundreds of expression‐dysregulated lnc RNA s in HCC tissues compared with normal tissues. Genomic copy number variation analysis showed that many of those lnc RNA s disorder are related to the copy number amplification or deletion. Moreover, several lnc RNA s expression levels are associated with HCC patients' overall and recurrence‐free survival, such as RP 1‐228H13.5, TMCC 1‐ AS 1, LINC 00205, and RP 11‐307C12.11. Furthermore, we identified two lnc RNA s termed PVT 1 and SNHG 7 that may be involved in HCC cells metastasis by comparing lnc RNA s expression profiles between early recurrence HCC tissues with metastasis and late recurrence HCC tissues without metastasis. Finally, loss‐of‐function assays confirmed that knockdown of SNHG 7 and PVT 1 impaired HCC cells invasion. Taken together, these findings may provide a valuable resource for further identification of novel biomarkers and therapeutic targets for HCC patients.