Open AccessLncRNA‐TCONS_00026907 is involved in the progression and prognosis of cervical cancer through inhibiting miR‐143‐5p
Author(s) -
Jin Xuejing,
Chen Xiangjian,
Hu Yan,
Ying Furong,
Zou Ruanmin,
Lin Feng,
Shi Zhengzheng,
Zhu Xuejie,
Yan Xiaojian,
Li Shi,
Zhu Hua
Publication year - 2017
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1084
Subject(s) - gene silencing , hela , cancer research , oncogene , apoptosis , cervical cancer , transfection , rna interference , small hairpin rna , microrna , cyclin d1 , cancer , rna , small interfering rna , chemistry , cell cycle , biology , microbiology and biotechnology , in vitro , medicine , gene , biochemistry
Our previous long noncoding RNA (lnc RNA ) microarray revealed that lnc RNA ‐ TCONS _00026907 is aberrantly expressed between cervical cancer tissues and adjacent tissues. This study aims to explore the potential role of TCONS _00026907 in the development of cervical cancer. The expression levels of TCONS _00026907 in cervical cancer tissues and adjacent tissues from 83 patients of cervical cancer were detected by quantitative real‐time polymerase chain reaction and the survival rate was analyzed. In vitro, HeLa and SiHa cells were transfected with small hairpin RNA (sh RNA )‐ TCONS _00026907, then cell proliferation, cycle distribution, apoptosis, migration and invasion were measured. To confirm TCONS _00026907 regulates expression of ELK 1 through inhibiting miR‐143‐5p, overexpression of miR‐143‐5p and silencing of ELK 1 were, respectively, performed in HeLa and SiHa cells. Results showed that TCONS _00026907 level was significantly higher in cervical cancer tissues compared to noncancerous tissues and the survival rate was lower in the high expression group. Silencing of TCONS _00026907, overexpression of miR‐143‐5p and silencing of ELK 1 inhibited cervical cell cycle, proliferation, migration, and invasion, but promoted apoptosis, respectively. Furthermore, silencing of TCONS _00026907 suppressed the growth of cervical tumors and altered the expression of ELK 1, p‐ ELK 1, C‐fos, Cyclin D1 and Bcl‐2 in vivo. Our study identifies TCONS _00026907 as a potent proto‐oncogene and indicates that TCONS _00026907/miR143‐5p/ ELK 1 regulatory pathway plays an important role in cervical cancer.